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细胞的微观世界有着复杂的运行规律。长期以来,人们很难看清其真实面貌。显微镜技术的发展进步,助力微观世界探索不断向纵深处发展。普通光学显微镜受可见光波长限制,分辨率只能达到约0.2微米,远不足以分辨蛋白质等纳米尺度的分子结构;传统电子显微镜虽然分辨率更高,却需要在真空环境中操作,样本必须脱水、染色并固定,导致生物分子失去天然构象,甚至被电子束灼烧破坏。1974年冷冻电镜技术的问世,带来了一场新的革命。